Molecular Biology Reagents and Kits
Biochain Institute Inc PureSil-Silica Magnetic Beads, 10 mL/pk
PureSil-Silica Magnetic Beads are Fe3O4 magnetic beads coated with a silicon dioxide (SiO2) layer. Since silica is able to bind to the nucleic acids, PureSil-Silica Magnetic Beads serve as a simple and efficient tool for plasmid DNA purification for transfection or sequencing applications, genomic DNA purification for research or clinical applications, RNA purification for qPCR analysis, or PCR product clean-up for downstream analysis.Features- Capacity: 4mg DNA/ml beads- 1-2 um particle size- Bead concentration: 4mg/ml- Bead density: ~1.03g/cm3- Simple, short procedure- No costly columns required- No time consuming centrifugations requiredApplications- Plasmid DNA isolation- Genomic DNA isolation- RNA purification for qPCR- PCR product clean up
Biochain Institute Inc Methylated & Low-Methylated DNA Matched Pair - Human Peripheral Blood Leukocyte, 2 x 5 ug/pk
The methylated and low-methylated matched pair DNA can be utilized as positive and negative controls,respectively, in research focusing on gene methylation studies. The low-methylated genomic DNAs are selected from human young donor blood peripheral leukocyte, liver tissue, or lymphocyte cell line. The DNAs derived from these sources naturally have a low level of methylation and display batch-to-batch consistency. Unlike DNAs from the DNA methyltransferase genetic knockout cell lines, these DNA sources are ideal for more natural control DNAs to be used in gene methylation studies.
Zymo Research Corporation ZymoBIOMICS™ DNA Binding Buffer, 250 mL
It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Macherey-Nagel MACHEREY-NAGEL NucleoBond Xtra Midi Columns, High Yield Ultrapure DNA, Fast 30 Min Prep, Package of 10 Columns
MACHEREY-NAGEL NucleoBond Xtra Midi Columns offer a rapid solution for isolating high-quality plasmid DNA through advanced anion-exchange chromatography. With integrated filters and a streamlined workflow, users can achieve ultrapure yields in approximately 30 minutes, significantly outperforming competitors. The unique silica-based resin ensures specific binding of plasmid DNA, making it ideal for sensitive applications like transfection and sequencing.
Zymo Research Corporation Quick-DNA/RNA™ MagBead (4 x 96 preps)
The extraction method has the option to recover total nucleic acids in one elution or DNA & RNA in separate elutions without the use of phenol.DNA/RNA is eluted into 50 l of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
New England Biolabs, Inc. NEBNext Ultra™ II Directional RNA Second Strand Synthesis Module – 96 reactions
The NEBNext Ultra II Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library preparation workflow. This workflow uses the dUTP method for strand-specific library construction, which depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix, included in this module.
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep with Sample Purification Beads – 24 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg. For convenience, this kit includes SPRISelect beads.
Roche Diagnostics KAPA Mouse Genotyping Kits - Standard kit, 500 reactions
KAPA Mouse Gentoyping Kits include KAPA Express Extract Enzyme (1 U/L), KAPA Express Extract Buffer (10X), and KAPA2G Fast (or Fast HotStart) Genotyping Mix (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs, and MgCl2 (at a final concentration of 1.5 mM). ReadyMixes for PCR are also sold separately. *This item is non returnable.
Roche Diagnostics KAPA mRNA HyperPrep Kit, 24 libraries
KAPA RNA HyperPrep Kits include all reagents required for RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups, while KAPA Adapters are available separately. Kits also include upfront depletion modules for mRNA capture (KK8580, KK8581), rRNA depletion (KK8560, KK8561), and rRNA and globin depletion (KK8562, KK8563). *This item is non returnable.
New England Biolabs, Inc. NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® – 24 reactions
The NEBNext Ultra II FS DNA Library Prep Kit for Illumina provides a fragmentation system: a fast and reliable solution for DNA fragmentation and library construction. A new DNA fragmentation reagent is combined with end repair and dA-tailing reagents, allowing these steps to be performed in the same tube, with no clean-ups or sample loss. The same fragmentation protocol is used for any input amount (100 pg–500 ng), or GC content. The kit also includes the NEBNext Ultra II Ligation Master Mix for adaptor ligation, and the NEBNext Ultra II Q5 Master Mix for uniform, high-fidelity library amplification.
- Perform fragmentation, end repair and dA-tailing with a single enzyme mix
- Experience reliable fragmentation with a single protocol, regardless of input amount or GC content
- Prepare high quality libraries from a wide range of input amounts: 100 pg-500 ng
- Use with DNA in standard buffers (TE, Tris-HCl) and water
Bioworld Endotoxin Removal Kit (Mini) - For DNA, 20 Prep(s)
For the rapid removal of endotoxins from previously purified DNA Reduce endotoxin levels to 0.1 EU/µg DNA or less Effectively remove endotoxins in as little as 20 minutes Remove endotoxins from up to 40 µg of DNA Rapid spin-column formatFEATURES AND BENEFITSFast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes Endotoxin-free plasmid DNA- Using Norgen’s Endotoxin Removal Kit (Mini), endotoxin levels are reduced to less than or equal to 0.1 EU/µg plasmid DNA High recovery of input DNA - Average DNA recovery is > 90% of the input amount Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with sequencing, cloning and transfections
Macherey-Nagel NucleoBond Xtra Combi Rack
NucleoBond Xtra Combi Rack rack for use with NucleoBond Xtra Midi, Xtra Maxi, Xtra BAC, AX 100, AX 500, AX 2000, and AX 10000 Columns pack of 1